Protein Assays

Protein Assays Below is a list of assays for the determination of protein concentration in a solution. This list includes the sensitivity range, volume/ touchstone of try enquireed, subjective comments on accuracy and convenience, and major interfering agents. adjective details, equipment requirements, and references are outlined in the individual assay documents. The criteria for selection of a protein assay are usually based on convenience, availability of protein for assay, presence or absence of interfering agents, and claim for accuracy. For example, the Lowry method is very sensitive just is a twain step procedure that requires a minimum of 40 proceedings incubation time. The Bradford assay is more sensitive and can be read within 5 minutes, however proteins with low arginine training will be underreckoningd. globally, estimates are more accurate for tough mixtures of proteins. Estimates of concentration of pure proteins can be very loose depending on th e principle of the assay, unless the same pure protein is used as a standard. Criteria will be discussed in the individual documents.

Because differentiate proteins have different amino acid compositions, the sensitivity of colorimetric assays to individual proteins may vary widely. The most reprodicible results are obtained with standards execute up of a mixture of proteins that is as similar as realistic to the unknown. For most purposes, a relative amount is total enough, but the standard used should be reported. For example, the Bradford assay is some(prenominal) more sensitive to bovine serum albumin (BSA) than to immune gamma globulin G (IgG)! , so that with IgG the investigator is likely to overestimation the amount of protein in a sample. With BSA the investigator is likely to underestimate the amount. General Reference: Stoscheck, CM. Quantitation of Protein. Methods in Enzymology 182: 50-69 (1990).If you want to get a broad essay, order it on our website: OrderCustomPaper.com

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